The intracellular sites of early replication and budding of SARS-coronavirus.
Identifieur interne : 003575 ( Main/Exploration ); précédent : 003574; suivant : 003576The intracellular sites of early replication and budding of SARS-coronavirus.
Auteurs : Silke Stertz [Allemagne] ; Mike Reichelt ; Martin Spiegel ; Thomas Kuri ; Luis Martínez-Sobrido ; Adolfo García-Sastre ; Friedemann Weber ; Georg KochsSource :
- Virology [ 0042-6822 ] ; 2007.
Descripteurs français
- KwdFr :
- ARN viral (métabolisme), Animaux, Appareil de Golgi (métabolisme), Cellules Vero, Cytoplasme (métabolisme), Cytoplasme (ultrastructure), Cytoplasme (virologie), Facteurs temps, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (métabolisme), Membrane cellulaire (métabolisme), Membrane cellulaire (ultrastructure), Membrane cellulaire (virologie), Microscopie immunoélectronique, Microscopie électronique, Protéines de l'enveloppe virale (métabolisme), Protéines nucléocapside (métabolisme), Protéines virales non structurales (métabolisme), RNA replicase (métabolisme), Réplication virale, Réticulum endoplasmique (métabolisme), Syndrome respiratoire aigu sévère (virologie), Transport de protéines, Virus du SRAS (physiologie), Vésicules cytoplasmiques (métabolisme).
- MESH :
- métabolisme : ARN viral, Appareil de Golgi, Cytoplasme, Glycoprotéines membranaires, Membrane cellulaire, Protéines de l'enveloppe virale, Protéines nucléocapside, Protéines virales non structurales, RNA replicase, Réticulum endoplasmique, Vésicules cytoplasmiques.
- physiologie : Virus du SRAS.
- virologie : Cytoplasme, Membrane cellulaire, Syndrome respiratoire aigu sévère.
- ultrastructure : Animaux, Cellules Vero, Cytoplasme, Facteurs temps, Glycoprotéine de spicule des coronavirus, Membrane cellulaire, Microscopie immunoélectronique, Microscopie électronique, Réplication virale, Transport de protéines.
English descriptors
- KwdEn :
- Animals, Cell Membrane (metabolism), Cell Membrane (ultrastructure), Cell Membrane (virology), Chlorocebus aethiops, Cytoplasm (metabolism), Cytoplasm (ultrastructure), Cytoplasm (virology), Cytoplasmic Vesicles (metabolism), Endoplasmic Reticulum (metabolism), Golgi Apparatus (metabolism), Membrane Glycoproteins (metabolism), Microscopy, Electron, Microscopy, Immunoelectron, Nucleocapsid Proteins (metabolism), Protein Transport, RNA Replicase (metabolism), RNA, Viral (metabolism), SARS Virus (physiology), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Time Factors, Vero Cells, Viral Envelope Proteins (metabolism), Viral Nonstructural Proteins (metabolism), Virus Replication.
- MESH :
- chemical , metabolism : Membrane Glycoproteins, Nucleocapsid Proteins, RNA Replicase, RNA, Viral, Viral Envelope Proteins, Viral Nonstructural Proteins.
- metabolism : Cell Membrane, Cytoplasm, Cytoplasmic Vesicles, Endoplasmic Reticulum, Golgi Apparatus.
- physiology : SARS Virus.
- ultrastructure : Cell Membrane, Cytoplasm.
- virology : Cell Membrane, Cytoplasm, Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Microscopy, Electron, Microscopy, Immunoelectron, Protein Transport, Spike Glycoprotein, Coronavirus, Time Factors, Vero Cells, Virus Replication.
Abstract
In this study, we analyzed the replication and budding sites of severe acute respiratory syndrome coronavirus (SARS-CoV) at early time points of infection. We detected cytoplasmic accumulations containing the viral nucleocapsid protein, viral RNA and the non-structural protein nsp3. Using EM techniques, we found that these putative viral replication sites were associated with characteristic membrane tubules and double membrane vesicles that most probably originated from ER cisternae. In addition to its presence at the replication sites, N also accumulated in the Golgi region and colocalized with the viral spike protein. Immuno-EM revealed that budding occurred at membranes of the ERGIC (ER-Golgi intermediate compartment) and the Golgi region as early as 3 h post infection, demonstrating that SARS-CoV replicates surprisingly fast. Our data suggest that SARS-CoV establishes replication complexes at ER-derived membranes. Later on, viral nucleocapsids have to be transported to the budding sites in the Golgi region where the viral glycoproteins accumulate and particle formation occurs.
DOI: 10.1016/j.virol.2006.11.027
PubMed: 17210170
Affiliations:
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sites of early replication and budding of SARS-coronavirus.</title><author><name sortKey="Stertz, Silke" sort="Stertz, Silke" uniqKey="Stertz S" first="Silke" last="Stertz">Silke Stertz</name><affiliation wicri:level="3"><nlm:affiliation>Department of Virology, University of Freiburg, D-79008 Freiburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Virology, University of Freiburg, D-79008 Freiburg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Reichelt, Mike" sort="Reichelt, Mike" uniqKey="Reichelt M" first="Mike" last="Reichelt">Mike Reichelt</name></author><author><name sortKey="Spiegel, Martin" sort="Spiegel, Martin" uniqKey="Spiegel M" first="Martin" last="Spiegel">Martin Spiegel</name></author><author><name sortKey="Kuri, Thomas" sort="Kuri, Thomas" uniqKey="Kuri T" first="Thomas" last="Kuri">Thomas Kuri</name></author><author><name sortKey="Martinez Sobrido, Luis" sort="Martinez Sobrido, Luis" uniqKey="Martinez Sobrido L" first="Luis" last="Martínez-Sobrido">Luis Martínez-Sobrido</name></author><author><name sortKey="Garcia Sastre, Adolfo" sort="Garcia Sastre, Adolfo" uniqKey="Garcia Sastre A" first="Adolfo" last="García-Sastre">Adolfo García-Sastre</name></author><author><name sortKey="Weber, Friedemann" sort="Weber, Friedemann" uniqKey="Weber F" first="Friedemann" last="Weber">Friedemann Weber</name></author><author><name sortKey="Kochs, Georg" sort="Kochs, Georg" uniqKey="Kochs G" first="Georg" last="Kochs">Georg Kochs</name></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Membrane (metabolism)</term><term>Cell Membrane (ultrastructure)</term><term>Cell Membrane (virology)</term><term>Chlorocebus aethiops</term><term>Cytoplasm (metabolism)</term><term>Cytoplasm (ultrastructure)</term><term>Cytoplasm (virology)</term><term>Cytoplasmic Vesicles (metabolism)</term><term>Endoplasmic Reticulum (metabolism)</term><term>Golgi Apparatus (metabolism)</term><term>Membrane Glycoproteins (metabolism)</term><term>Microscopy, Electron</term><term>Microscopy, Immunoelectron</term><term>Nucleocapsid Proteins (metabolism)</term><term>Protein Transport</term><term>RNA Replicase (metabolism)</term><term>RNA, Viral (metabolism)</term><term>SARS Virus (physiology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Time Factors</term><term>Vero Cells</term><term>Viral Envelope Proteins (metabolism)</term><term>Viral Nonstructural Proteins (metabolism)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (métabolisme)</term><term>Animaux</term><term>Appareil de Golgi (métabolisme)</term><term>Cellules Vero</term><term>Cytoplasme (métabolisme)</term><term>Cytoplasme (ultrastructure)</term><term>Cytoplasme (virologie)</term><term>Facteurs temps</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Membrane cellulaire (métabolisme)</term><term>Membrane cellulaire (ultrastructure)</term><term>Membrane cellulaire (virologie)</term><term>Microscopie immunoélectronique</term><term>Microscopie électronique</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Protéines nucléocapside (métabolisme)</term><term>Protéines virales non structurales (métabolisme)</term><term>RNA replicase (métabolisme)</term><term>Réplication virale</term><term>Réticulum endoplasmique (métabolisme)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Transport de protéines</term><term>Virus du SRAS (physiologie)</term><term>Vésicules cytoplasmiques (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>RNA Replicase</term><term>RNA, Viral</term><term>Viral Envelope Proteins</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term><term>Cytoplasm</term><term>Cytoplasmic Vesicles</term><term>Endoplasmic Reticulum</term><term>Golgi Apparatus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term><term>Appareil de Golgi</term><term>Cytoplasme</term><term>Glycoprotéines membranaires</term><term>Membrane cellulaire</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Protéines virales non structurales</term><term>RNA replicase</term><term>Réticulum endoplasmique</term><term>Vésicules cytoplasmiques</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Cell Membrane</term><term>Cytoplasm</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cytoplasme</term><term>Membrane cellulaire</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cell Membrane</term><term>Cytoplasm</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Microscopy, Electron</term><term>Microscopy, Immunoelectron</term><term>Protein Transport</term><term>Spike Glycoprotein, Coronavirus</term><term>Time Factors</term><term>Vero Cells</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Cytoplasme</term><term>Facteurs temps</term><term>Glycoprotéine de spicule des coronavirus</term><term>Membrane cellulaire</term><term>Microscopie immunoélectronique</term><term>Microscopie électronique</term><term>Réplication virale</term><term>Transport de protéines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, we analyzed the replication and budding sites of severe acute respiratory syndrome coronavirus (SARS-CoV) at early time points of infection. We detected cytoplasmic accumulations containing the viral nucleocapsid protein, viral RNA and the non-structural protein nsp3. Using EM techniques, we found that these putative viral replication sites were associated with characteristic membrane tubules and double membrane vesicles that most probably originated from ER cisternae. In addition to its presence at the replication sites, N also accumulated in the Golgi region and colocalized with the viral spike protein. Immuno-EM revealed that budding occurred at membranes of the ERGIC (ER-Golgi intermediate compartment) and the Golgi region as early as 3 h post infection, demonstrating that SARS-CoV replicates surprisingly fast. Our data suggest that SARS-CoV establishes replication complexes at ER-derived membranes. Later on, viral nucleocapsids have to be transported to the budding sites in the Golgi region where the viral glycoproteins accumulate and particle formation occurs.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>District de Fribourg-en-Brisgau</li></region><settlement><li>Fribourg-en-Brisgau</li></settlement></list><tree><noCountry><name sortKey="Garcia Sastre, Adolfo" sort="Garcia Sastre, Adolfo" uniqKey="Garcia Sastre A" first="Adolfo" last="García-Sastre">Adolfo García-Sastre</name><name sortKey="Kochs, Georg" sort="Kochs, Georg" uniqKey="Kochs G" first="Georg" last="Kochs">Georg Kochs</name><name sortKey="Kuri, Thomas" sort="Kuri, Thomas" uniqKey="Kuri T" first="Thomas" last="Kuri">Thomas Kuri</name><name sortKey="Martinez Sobrido, Luis" sort="Martinez Sobrido, Luis" uniqKey="Martinez Sobrido L" first="Luis" last="Martínez-Sobrido">Luis Martínez-Sobrido</name><name sortKey="Reichelt, Mike" sort="Reichelt, Mike" uniqKey="Reichelt M" first="Mike" last="Reichelt">Mike Reichelt</name><name sortKey="Spiegel, Martin" sort="Spiegel, Martin" uniqKey="Spiegel M" first="Martin" last="Spiegel">Martin Spiegel</name><name sortKey="Weber, Friedemann" sort="Weber, Friedemann" uniqKey="Weber F" first="Friedemann" last="Weber">Friedemann Weber</name></noCountry><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Stertz, Silke" sort="Stertz, Silke" uniqKey="Stertz S" first="Silke" last="Stertz">Silke Stertz</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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